peg bacmam expression vector Search Results


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Image Search Results


Journal: eLife

Article Title: Structure of the human heparan-α-glucosaminide N -acetyltransferase (HGSNAT)

doi: 10.7554/eLife.93510

Figure Lengend Snippet:

Article Snippet: The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683) between EcoRI and NotI restriction sites, to be expressed via baculoviral transduction in HEK293S GnTI - cells (ATCC # CRL-3022) as a fusion protein containing an N-terminal Strep-tag-II-GFP.

Techniques: Sequencing, Expressing, Transfection, Construct, Recombinant, Plasmid Preparation, Cloning, Modification, Software, Cell Culture, Size-exclusion Chromatography, Affinity Purification, Electron Microscopy